Anti-V5 tag Antibody

Cat: 100378-MM04
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Anti-V5 tag Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-V5 tag Antibody
Validated applications
ICC/IF,IF
Specificity
V5 tag
Immunogen
A synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST).
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #04
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-V5 tag Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ICC-IF 1:20-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-V5 tag Antibody (Mouse Monoclonal antibody) Images
  Immunohistochemistry(IHC) 2739
Immunofluorescence staining of V5-Tag in Hela cells, transfected with pSTEP2-V5-mFABP4-his (Figure A), pSTEP2-mFABP4-V5-his (Figure B), pSTEP2-V5-ARG1-his (Figure C) and pSTEP2-his-ARG1-V5 (Figure D). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-flag-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
Product Description Host Clonality Application Catalog# (PDF)
Anti-V5 tag Antibody Mouse Monoclonal WB 100378-MM03
Anti-V5 tag Antibody Mouse Monoclonal WB,IP 100378-MM01
Anti-V5 tag Antibody Rabbit Polyclonal WB,IP 100378-T36
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