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Anti-V5 標籤 抗體, 鼠單抗

  • Anti-V5 Tag Antibody, Mouse MAb, Immunofluorescence
產品資料評論相關產品實驗方法
 V5 抗體產品資訊
免疫原:A synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST).
Clone ID:04
Ig類型:Mouse IgG1
濃度:
內毒素:
緩衝液:0.2 μm filtered solution in PBS
抗體製備方法:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 V5 Antibody IF Application Image
Anti-V5 Tag Antibody, Mouse MAb, Immunofluorescence
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Caption:
Immunofluorescence staining of V5-Tag in Hela cells, transfected with pSTEP2-V5-mFABP4-his (Figure A), pSTEP2-mFABP4-V5-his (Figure B), pSTEP2-V5-ARG1-his (Figure C) and pSTEP2-his-ARG1-V5 (Figure D). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-flag-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
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貨號: 100378-MM04-50
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Datasheet & Documentation

All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
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