Anti-S100B Antibody

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Anti-S100B Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-S100B Antibody
Validated applications
IHC-P
Species reactivity
Reacts with: Human
Specificity
Human S100B
Immunogen
Recombinant Human S100B Protein (Catalog#10181-H07E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human S100B (rh S100B; Catalog#10181-H07E; NP_006263.1; Ser2-Glu92). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2a Clone #60
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-S100B Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
IHC-P 1:200-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-S100B Antibody (Mouse Monoclonal antibody) Images

Immunochemical staining of human S100B in human malignant melanoma with mouse monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human S100B in human lymphoma with mouse monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Anti-S100B Antibody: Alternative Names

Anti-NEF Antibody; Anti-S100 Antibody; Anti-S100-B Antibody; Anti-S100beta Antibody

S100B Background Information

S1B is a member of the S1 family of proteins containing two EF-hand-type calcium-binding motifs. S1B exerts both intracellular and extracellular functions. Intracellular S1B acts as a stimulator of cell proliferation and migration and an inhibitor of apoptosis and differentiation, which might have important implications during brain, cartilage and skeletal muscle development and repair, activation of astrocytes in the course of brain damage and neurodegenerative processes, and of cardiomyocyte remodeling after infarction, as well as in melanomagenesis and gliomagenesis. As an extracellular factor, S1B engages RAGE (receptor for advanced glycation end products) in a variety of cell types with different outcomes (i.e. beneficial or detrimental, pro-proliferative or pro-differentiative) depending on the concentration attained by the protein, the cell type and the microenvironment. This calcium binding astrocyte-specific cytokine, presents a marker of astrocytic activation and reflects CNS injury. The excellent sensitivity of S1B has enabled it to confirm the existence of subtle brain injury in patients with mild head trauma, strokes, and after successful resuscitation from cardiopulmonary arrest. Recent findings provide evidence, that S1B may decrease neuronal injury and/or contribute to repair following traumatic brain injury (TBI). Hence, S1B, far from being a negative determinant of outcome, as suggested previously in the human TBI and ischemia literature, is of potential therapeutic value that could improve outcome in patients who sustain various forms of acute brain damage.
Full Name
S100 calcium binding protein B
References
  • Kleindienst A, et al. (2006) A critical analysis of the role of the neurotrophic protein S100B in acute brain injury. J Neurotrauma. 23(8): 1185-200.
  • Bloomfield SM, et al. (2007) Reliability of S100B in predicting severity of central nervous system injury. Neurocrit Care. 6(2): 121-38.
  • Donato R, et al. (2009) S100B's double life: intracellular regulator and extracellular signal. Biochim Biophys Acta. 1793(6): 1008-22.
  • Beaudeux JL. (2009) S100B protein: a novel biomarker for the diagnosis of head injury. Ann Pharm Fr. Beaudeux JL. 67(3): 187-94.

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Mouse MAb

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