MERS-CoV (NCoV / Novel coronavirus) Spike Protein S1 Antibody, Rabbit PAb, Antigen Affinity Purified

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MERS-CoV (NCoV / Novel coronavirus) Spike Protein S1 Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal antibody) General Information

Product name
MERS-CoV (NCoV / Novel coronavirus) Spike Protein S1 Antibody, Rabbit PAb, Antigen Affinity Purified
Validated applications
WB,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Application notes
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
MERS-CoV CoV spike
Immunogen
A synthetic peptide corresponding to the center region of the S1 subunit of MERS-CoV (NCoV / Novel coronavirus) spike glycoprotein (S protein).
Preparation
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the center region of the S1 subunit of MERS-CoV (NCoV / Novel coronavirus) spike glycoprotein (S protein), and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

MERS-CoV (NCoV / Novel coronavirus) Spike Protein S1 Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

MERS-CoV (NCoV / Novel coronavirus) Spike Protein S1 Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal antibody) Images

Anti-MERS-coV(NCoV/Novel coronavirus)Spike Protein S1 rabbit polyclonal antibody at 1:500 dilution.

Sample:Novel coronavirus (HCoV-EMC/2012) Spike protein (ECD, aa 1-1297)

Lane A: 500ng

Lane B: 200ng

Lane C: 100ng

Secondary

Goat Anti- Rabbit IgG (H+L)/HRP at 0.4ug/mldilution.

Performed under reducing conditions.

MERS-CoV (NCoV / Novel coronavirus) Spike Protein S1 Antibody, Rabbit PAb, Antigen Affinity Purified: Alternative Names

Anti-S Antibody

CoV spike Background Information

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.
References
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.

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