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Anti-E-tag 抗體, 兔多抗, 抗原親和純化

  • Anti-E-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Western Blot
  • Anti-E-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Immunohistochemistry
  • Anti-E-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Immunoprecipitation
產品資料評論相關產品實驗方法
  抗體產品資訊
免疫原:A synthetic peptide corresponding to the E-tag sequence (GAPVPYPDPLEPR).
Clone ID:
Ig類型:Rabbit IgG
濃度:
內毒素:
緩衝液:0.2 μm filtered solution in PBS
抗體製備方法:Produced in rabbits immunized with A synthetic peptide corresponding to the E-tag sequence (GAPVPYPDPLEPR), and purified by antigen affinity chromatography.
  Antibody WB Application Image
  Antibody IF Application Image
Anti-E-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Immunohistochemistry
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Caption:
Immunofluorescence staining of E-Tag in Hela cells, transfected with pSTEP2-Etag-GFERh (Figure A), pSTEP2-his-Etag-GFER (Figure B), pSTEP2-his-GFER-Etag (Figure C), pSTEP2-Etag-GSTT2h (Figure D),pSTEP2-his-Etag-GSTT2 (Figure E) and pSTEP2-his-GSTT2-Etag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-E-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
  Antibody IP Application Image
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