Anti-DYKDDDDK (FLAG® epitope Tag) Antibody

Cat: 100233-MM01
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All DYKDDDDK (FLAG® epitope Tag) Reagents
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All DYKDDDDK (FLAG® epitope Tag) Reagents
Anti-DYKDDDDK (FLAG® epitope Tag) Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-DYKDDDDK (FLAG® epitope Tag) Antibody
Validated applications
DYKDDDDK (FLAG® epitope Tag)
A synthetic peptide corresponding to the flag-tag sequence (DYKDDDDK).
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, the synthetic peptide corresponding to the DYKDDDDK-tag sequence (CDYKDDDDK). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG2b Clone #01
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-DYKDDDDK (FLAG® epitope Tag) Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ICC-IF 1:50-1:1000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-DYKDDDDK (FLAG® epitope Tag) Antibody (Mouse Monoclonal antibody) Images
 FLAG Immunofluorescence(IF) 3047
Immunofluorescence staining of flag-Tag in 293 cells, transfected with PCMV-CDH1-flag (Figure A), pSTEP2-Flag-FABP4-GST (Figure B), pSTEP2-Flag-ARG1-GST (Figure C), Flag-PRMT5-His (Figure D),Flag-PRMT6-His (Figure E) and His-flag-CD38(Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-flag-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
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