输入关键词搜索之后,可以在左侧选择分子或者产品类型以精简过滤器,快速查找产品。如果有任何问题,请 联系我们!

快速訂購

Text Size:AAA

人 AK4 / Adenylate Kinase 4 / AK3L1 基因全長cDNA ORF(克隆載體)

產品資料評論相關產品實驗方法
Human AK4/AK3L1 cDNA Clone產品資訊
註冊碼:BC016180
cDNA基因長度:672bp
cDNA基因描述:Full length Clone DNA of Homo sapiens adenylate kinase 4.
基因別名:AK3, AK3L1, AK3L2
分子種屬:Human
載體:pGEM-T Vector
Plasmid:pGEM-AK3L1
限制性酶切位點:
Tag序列:
序列資訊:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
人 AK4 / Adenylate Kinase 4 / AK3L1 基因全長cDNA ORF(克隆載體) on other vectors
Product nameProduct name
研究背景

Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member the adenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 ( AK4 / AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.

Size / Price
貨號: HG12406-G
目錄價: 
單價:      (You Save: )
規格:
數量:+-
庫存In Stock
大包裝查詢加入購物車
Contact Us
產品圖片
      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
      請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”