AK4 was immunoprecipitated using:
Lane A:0.5 mg HepG2 Whole Cell Lysate
Lane B:0.5 mg A549 Whole Cell Lysate
Lane C:0.5 mg MCF-7 Whole Cell Lysate0.5 µL anti-AK4 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-AK4 mouse monoclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 26 kDa
Observed band size: 26 kDa
Anti-AK4 mouse monoclonal antibody at 1:500 dilution
Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:26 kDa
Observed band size:26 kDa