Anti-ABHD14B Antibody

Cat: 13913-R011

Contact us:

215-583-7898
order@sinobiological.com
Distributors

All ABHD14B Reagents

CRO Services

Product recommended


All ABHD14B Reagents

Anti-ABHD14B Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-ABHD14B Antibody
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human ABHD14B
Immunogen
Recombinant Human ABHD14B protein (Catalog#13913-H07E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ABHD14B (rh ABHD14B; Catalog#13913-H07E; Q96IU4-1; Met1-Gln210).
Source
Monoclonal Rabbit IgG Clone #011
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-ABHD14B Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:1000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-ABHD14B Antibody (Rabbit Monoclonal antibody) Images

Human ABHD14B Western blot (WB) 6695

Anti-ABHD14B rabbit monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:22 kDa

Observed band size:23 kDa

Human Pancreasin/Marapsin/PRSS27 Immunoprecipitation(IP) 8125

ABHD14B was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

0.5 µL anti-ABHD14B rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ABHD14B rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 25 kDa

Observed band size: 25 kDa

Anti-ABHD14B Antibody Alternative Names

Anti-CIB Antibody;Anti-HEL-S-299 Antibody

ABHD14B Background Information

ABHD14B belongs to the AB hydrolase superfamily, ABHD14 family. It can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. ABHD14B contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members.

Full Name
abhydrolase domain containing 14B
References
  • Mehrle A, et al. (2006) The LIFEdb database in 2006. Nucleic Acids Res. 34:D415-8.
  • Wan D, et al. (2004) Large-scale cDNA transfection screening for genes related to cancer development and progression. Proc Natl Acad Sci. 101(44):15724-9.
  • Wiemann S, et al. (2004) From ORFeome to biology: a functional genomics pipeline. Genome Res. 14 (10B):2136-44.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-ABHD14B Antibody Rabbit Polyclonal ELISA 13913-RP01
    Anti-ABHD14B Antibody Rabbit Monoclonal ELISA,ICC/IF,IF 13913-R002
    Anti-ABHD14B Antibody Rabbit Polyclonal ELISA,IHC-P 13913-T24
    info info
    添加购物车成功! 添加购物车失败!