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ABHD10  基因

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HP103087 

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ABHD10 相关研究领域

ABHD10 相關信號通路

    ABHD10 概述&蛋白信息

    ABHD10 研究背景

    基因概述: ABHD10 gene encodes a mitochondrially-localized enzyme that acts in liver cells as a hydrolase. The encoded protein removes glucuronide from mycophenolic acid acyl-glucuronide. There is a pseudogene for this gene on chromosome 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
    General information above from NCBI
    催化活性: Mycophenolic acid O-acyl-glucuronide + H(2)O = mycophenolate + D-glucuronate. {ECO:0000269|PubMed:22294686}.
    亞細胞定位: Mitochondrion {ECO:0000305}.
    相似的序列: Belongs to the AB hydrolase superfamily. {ECO:0000305}.
    General information above from UniProt

    Mycophenolic acid (MPA), the active metabolite of the immunosuppressant mycophenolate mofetil (MMF), is primarily metabolized by glucuronidation to a phenolic glucuronide (MPAG) and an acyl glucuronide (AcMPAG). It is known that AcMPAG, which may be an immunotoxic metabolite, is deglucuronidated in human liver. AcMPAG deglucuronidation activity was detected in both human liver cytosol (HLC) and microsomes (HLM). By purification from HLC with column chromatographic purification steps, the enzyme responsible for AcMPAG deglucuronidationis identified as α/β hydrolase domain containing 10 (ABHD10). Recombinant ABHD10 expressed in Sf9 cells efficiently deglucuronidated AcMPAG with a K(m) value of 100.7 ± 10.2 μM, which was similar to those in HLM, HLC, and human liver homogenates (HLH). Immunoblot analysis revealed ABHD10 protein expression in both HLC and HLM. The AcMPAG deglucuronidation by recombinant ABHD10, HLC, and HLH were potently inhibited by AgNO(3), CdCl(2), CuCl(2), PMSF, bis-p-nitrophenylphosphate, and DTNB. The CL(int) value of AcMPAG formation from MPA, which was catalyzed by human UGT2B7, in HLH was increased by 1.8-fold in the presence of PMSF. Thus, human ABHD10 would affect the formation of AcMPAG, the immunotoxic metabolite.

    ABHD10 別稱

    mitochondrial,ABHD10,abhydrolase domain containing 10,abhydrolase domain-containing protein 10, [human]
    mitochondrial,Abhd10,abhydrolase domain containing 10,abhydrolase domain-containing protein 10,D230019K24Rik,D630018L06, [mouse]

    ABHD10 相關文獻

  • Nardini M. et al., 1999, Curr Opin Struct Biol. 9 (6): 732-7.
  • Carr PD. et al., 2009, Protein Pept Lett. 16 (10): 1137-48.
  • Cheah E. et al., 1992, Protein Eng. 5 (3): 197-211.
  • Iwamura A. et al., 2012, J Biol Chem. 287 (12): 9240-9.
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