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人 ABHD10 蛋白 (aa 53-306, His 標籤)

  • Human ABHD10 Protein (aa 53-306, His Tag) SDS-PAGE
產品資料評論相關產品實驗方法
 ABHD10 蛋白產品資訊
分子別稱:ABHD10
蛋白構建:A DNA sequence encoding the human ABHD10 (Q9NUJ1)(Thr53-Asn306) was fused with a polyhistide tag at the N-terminus.
分子種屬:Human
表達宿主:Baculovirus-Insect Cells
#N/A:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
 ABHD10 蛋白質控檢測
純度:> 90 % as determined by SDS-PAGE
內毒素:< 1.0 EU per μg of the protein as determined by the LAL method
穩定性:Samples are stable for up to twelve months from date of receipt at -70℃
預測N端:His
分子量:The recombinant human ABHD10 consists of 271 amino acids and has a calculated molecular mass of 30.3 kDa. The recombinant protein migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.
緩衝液:Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
 ABHD10 蛋白使用指導
儲存方法:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
#N/A:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
 ABHD10 Protein SDS-PAGE
Human ABHD10 Protein (aa 53-306, His Tag) SDS-PAGE

ABHD10 相關信號通路

    Other ABHD10 Recombinant Protein Products
    14439-H07B
    ABHD10 研究背景

    Mycophenolic acid (MPA), the active metabolite of the immunosuppressant mycophenolate mofetil (MMF), is primarily metabolized by glucuronidation to a phenolic glucuronide (MPAG) and an acyl glucuronide (AcMPAG). It is known that AcMPAG, which may be an immunotoxic metabolite, is deglucuronidated in human liver. AcMPAG deglucuronidation activity was detected in both human liver cytosol (HLC) and microsomes (HLM). By purification from HLC with column chromatographic purification steps, the enzyme responsible for AcMPAG deglucuronidationis identified as α/β hydrolase domain containing 10 (ABHD10). Recombinant ABHD10 expressed in Sf9 cells efficiently deglucuronidated AcMPAG with a K(m) value of 100.7 ± 10.2 μM, which was similar to those in HLM, HLC, and human liver homogenates (HLH). Immunoblot analysis revealed ABHD10 protein expression in both HLC and HLM. The AcMPAG deglucuronidation by recombinant ABHD10, HLC, and HLH were potently inhibited by AgNO(3), CdCl(2), CuCl(2), PMSF, bis-p-nitrophenylphosphate, and DTNB. The CL(int) value of AcMPAG formation from MPA, which was catalyzed by human UGT2B7, in HLH was increased by 1.8-fold in the presence of PMSF. Thus, human ABHD10 would affect the formation of AcMPAG, the immunotoxic metabolite.

     ABHD10 參考資料
  • Nardini M. et al., 1999, Curr Opin Struct Biol. 9 (6): 732-7.
  • Carr PD. et al., 2009, Protein Pept Lett. 16 (10): 1137-48.
  • Cheah E. et al., 1992, Protein Eng. 5 (3): 197-211.
  • Iwamura A. et al., 2012, J Biol Chem. 287 (12): 9240-9.
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    貨號: 14439-H07B-100
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    Datasheet & Documentation

    Contact Us
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”