Anti-AARSD1 Antibody

Cat: 14280-T52
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Anti-AARSD1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-AARSD1 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human AARSD1
Immunogen
Recombinant Human AARSD1 protein (Catalog#14280-H07E)
Preparation
Produced in rabbits immunized with purified, recombinant Human AARSD1 (rh AARSD1; Catalog#14280-H07E; Q9BTE6-2; Thr176-Glu525). AARSD1 specific IgG was purified by Human AARSD1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-AARSD1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-AARSD1 Antibody (Rabbit Polyclonal antibody) Images
Human AARSD1 Immunoprecipitation(IP) 6066

AARSD1 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

2 µL anti-AARSD1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-AARSD1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 45 kDa

Observed band size: 45 kDa

Human AARSD1 Western blot (WB) 6711

Anti-AARSD1 rabbit polyclonal antibody at 1:500 dilution

Lane A: HeLa Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:45 kDa

Observed band size:45 kDa

(We are unsure as to the identity of these extra bands.)

Anti-AARSD1 Antibody Alternative Names
Anti-AARSD1 Antibody
AARSD1 Background Information

AARSD1 belongs to the class-II aminoacyl-tRNA synthetase family, Alax-L subfamily. AARSD1 binds 1 zinc ion per subunit functions in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala). Four transcript variants have been described for AARSD1: NM_025267.3, NM_001136042.2, NM_001142653.1 and NM_001142654.1. It has been determined that the latter two variants represent a distinct upstream locus, which is now represented by GeneID:100885848 (PTGES3L), while the former two variants represent readthrough transcripts between PTGES3L and this locus (AARSD1). The readthrough locus (PTGES3L-AARSD1) is now represented by GeneID:100885850.

Full Name
alanyl-tRNA synthetase domain containing 1
References
  • Tatham MH, et al. (2011) Comparative proteomic analysis identifies a role for SUMO in protein quality control. Oncogene. 19(17):2120-8.
  • Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40.
  • Udeshi ND, et al. (2012) Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. Mol Cell Proteomics. 11(5):148-59.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-AARSD1 Antibody Mouse Monoclonal ELISA 14280-MM01
    Anti-AARSD1 Antibody Rabbit Monoclonal WB,ELISA,ICC/IF,IF 14280-R012
    Anti-AARSD1 Antibody Rabbit Polyclonal ELISA 14280-RP01
    info info
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