|Recombinant Human AARSD1 protein (Catalog#14280-H07E)|
|0.2 μm filtered solution in PBS|
|Produced in rabbits immunized with purified, recombinant Human AARSD1 (rh AARSD1; Catalog#14280-H07E; Q9BTE6-2; Thr176-Glu525). AARSD1 specific IgG was purified by Human AARSD1 affinity chromatography.|
WB: 2-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human AARSD1. The detection limit for Human AARSD1 is < 0.039 ng/well.
IP: 1-4 μg/mg of lysate
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
AARSD1 belongs to the class-II aminoacyl-tRNA synthetase family, Alax-L subfamily. AARSD1 binds 1 zinc ion per subunit functions in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala). Four transcript variants have been described for AARSD1: NM_025267.3, NM_001136042.2, NM_001142653.1 and NM_001142654.1. It has been determined that the latter two variants represent a distinct upstream locus, which is now represented by GeneID:100885848 (PTGES3L), while the former two variants represent readthrough transcripts between PTGES3L and this locus (AARSD1). The readthrough locus (PTGES3L-AARSD1) is now represented by GeneID:100885850.