Text Size:AAA
留言    收藏

人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF(克隆載體)

    產品資料評論相關產品實驗方法
     AARS cDNA Clone產品資訊
    註冊碼:NM_001605.2
    cDNA基因長度:2907bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens alanyl-tRNA synthetase.
    基因別名:AARS
    分子種屬:Human
    載體:pMD18-T Simple Vector
    Plasmid:pMD-AARS
    限制性酶切位點:
    Tag序列:
    序列資訊:Identical with the Gene Bank Ref. ID sequence.
    Sequencing primers:M13-47 and RV-M
    ( We provide with AARS qPCR primers for gene expression analysis, HP100828 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    運輸條件:Each tube contains lyophilized plasmid.
    儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
    pMD18-T Simple Vector Information

    pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

    pMD18-T Simple Usage Suggestion

    The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

    Vector Sequence Download
    人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF(克隆載體) on other vectors
    人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG10951-ACGNT$11770
    人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG10951-ACRNT$11770
    人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤HG10951-ANGNT$11770
    人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤HG10951-ANRNT$11770
    人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10951-CFNT$10690
    人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG10951-CHNT$10690
    人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG10951-CMNT$10690
    人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG10951-CYNT$10690
    人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF克隆 (表達載體)HG10951-M-NNT$10690
    人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG10951-NFNT$10690
    人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG10951-NHNT$10690
    人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG10951-NMNT$10690
    人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG10951-NYNT$10690
    人 AARS / alanyl-tRNA synthetase 基因全長cDNA ORF克隆 (表達載體)HG10951-UTNT$10690
     點擊瞭解更多載體資訊
    Product nameProduct name
    研究背景

    Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections. 

    參考資料
  • Wakasugi K, et al. (1999) Two Distinct Cytokines Released from a Human Aminoacyl-tRNA Synthetase. Science. 284 (5411): 147-51.
  • Sokabe M, et al. (2009) The structure of alanyl-tRNA synthetase with editing domain. Proc Natl Acad Sci . 106 (27): 11028-33.
  • Skupinska M, et al. (2009) AARS--the etiological factor and the attractive target of many disorders. Postepy Biochem. 55 (4): 373-84.
  • Size / Price
    貨號: HG10951-M
    目錄價: 
    單價:      (You Save: )
    規格:
    數量:+-
    庫存In Stock
    加入購物車大包裝查詢

    Datasheet & Documentation

    Contact Us
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”