Anti-alanyl-tRNA synthetase Antibody

Cat: 50384-T60

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Anti-alanyl-tRNA synthetase Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-alanyl-tRNA synthetase Antibody
Validated applications
WB,ELISA,IHC-P,ICC/IF,IF,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse alanyl-tRNA synthetase
Immunogen
Recombinant Mouse AARS / alanyl-tRNA synthetase Protein (Catalog#50384-M08B)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse AARS / alanyl-tRNA synthetase (rM AARS / alanyl-tRNA synthetase; Catalog#50384-M08B; Q8BGQ7; Met1-Asn968). AARS / alanyl-tRNA synthetase specific IgG was purified by Mouse AARS / alanyl-tRNA synthetase affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-alanyl-tRNA synthetase Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IHC-P 1:2500-1:15000  
ICC-IF 1:1000-1:5000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-alanyl-tRNA synthetase Antibody (Rabbit Polyclonal antibody) Images

Mouse AARS / alanyl-tRNA synthetase Immunohistochemistry(IHC) 3089
Immunofluorescence staining of AARS in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-mouse AARS polyclonal antibody (1:5000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Mouse AARS / alanyl-tRNA synthetase Immunohistochemistry(IHC) 3403
Immunochemical staining of mouse AARS in mouse stomach with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.
Mouse AARS / alanyl-tRNA synthetase Immunohistochemistry(IHC) 3404
Immunochemical staining of mouse AARS in mouse kidney with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.
Mouse AARS / alanyl-tRNA synthetase Immunoprecipitation(IP) 6090

Mouse AARS was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

0.5 µL anti-Mouse AARS rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-Mouse AARS rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 107 kDa

Observed band size: 107 kDa

Mouse AARS / alanyl-tRNA synthetase Western blot (WB) 6773

Anti-AARS rabbit polyclonal antibody at 1:500 dilution

Lane A: NIH3T3 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:107 kDa

Observed band size:107 kDa

(We are unsure as to the identity of these extra bands.)

Anti-alanyl-tRNA synthetase Antibody Alternative Names

Anti-AI316495 Antibody;Anti-C76919 Antibody;Anti-sti Antibody

alanyl-tRNA synthetase Background Information

Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections.
Full Name
alanyl-tRNA synthetase
References
  • Wakasugi K, et al. (1999) Two Distinct Cytokines Released from a Human Aminoacyl-tRNA Synthetase. Science. 284 (5411): 147-51.
  • Sokabe M, et al. (2009) The structure of alanyl-tRNA synthetase with editing domain. Proc Natl Acad Sci . 106 (27): 11028-33.
  • Skupinska M, et al. (2009) AARS--the etiological factor and the attractive target of many disorders. Postepy Biochem. 55 (4): 373-84.
  • Product Description Host Clonality Application Catalog# (PDF)
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