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人 14-3-3 sigma/Stratifin/YWHAS 基因全長cDNA ORF(克隆載體)

    產品資料評論相關產品實驗方法
     YWHAS/SFN cDNA Clone產品資訊
    註冊碼:NM_006142.3
    cDNA基因長度:747bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens stratifin.
    基因別名:YWHAS, SFN
    分子種屬:Human
    載體:pMD18-T Simple Vector
    Plasmid:pMD-SFN
    限制性酶切位點:
    Tag序列:
    序列資訊:Identical with the Gene Bank Ref. ID sequence except for the point mutation 746 G/A not causing the amino acid variation.
    Sequencing primers:M13-47 and RV-M
    ( We provide with SFN qPCR primers for gene expression analysis, HP100737 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    運輸條件:Each tube contains lyophilized plasmid.
    儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
    pMD18-T Simple Vector Information

    pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

    pMD18-T Simple Usage Suggestion

    The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

    Vector Sequence Download
    人 14-3-3 sigma/Stratifin/YWHAS 基因全長cDNA ORF(克隆載體) on other vectors
    人 14-3-3 sigma/Stratifin/YWHAS 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG10838-ACGNT$8150
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    人 14-3-3 sigma/Stratifin/YWHAS 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG10838-NFNT$7060
    人 14-3-3 sigma/Stratifin/YWHAS 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG10838-NHNT$7060
    人 14-3-3 sigma/Stratifin/YWHAS 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG10838-NMNT$7060
    人 14-3-3 sigma/Stratifin/YWHAS 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG10838-NYNT$7060
    人 14-3-3 sigma/Stratifin/YWHAS 基因全長cDNA ORF克隆 (表達載體)HG10838-UTNT$7060
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    研究背景

    14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

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    貨號: HG10838-M
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