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人 14-3-3 epsilon / YWHAE 蛋白 (GST 標籤)

  • Human 14-3-3 epsilon / YWHAE Protein (GST Tag) SDS-PAGE
產品資料評論相關產品實驗方法
 YWHAE 蛋白產品資訊
分子別稱:14-3-3E,HEL2,KCIP-1,MDCR,MDS
蛋白構建:A DNA sequence encoding the human YWHAE (NP_006752.1) (Met 1-Gln 255) was fused with the GST tag at the N-terminus.
分子種屬:Human
表達宿主:E. coli
#N/A:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
 YWHAE 蛋白質控檢測
純度:> 92 % as determined by SDS-PAGE
內毒素:Please contact us for more information.
穩定性:Samples are stable for up to twelve months from date of receipt at -70℃
預測N端:Met
分子量:The recombinant human YWHAE/GST chimera consists of 487 amino acids and has a predicted molecular mass of 56 kDa. It migrates as an approxiamtely 50 kDa band in SDS-PAGE under reducing conditions.
緩衝液:Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.25mM DTT, 25% glycerol, 0.5mM GSH, pH 7.5
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
 YWHAE 蛋白使用指導
儲存方法:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
#N/A:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
 YWHAE Protein SDS-PAGE
Human 14-3-3 epsilon / YWHAE Protein (GST Tag) SDS-PAGE
Other YWHAE Recombinant Protein Products
10842-H09E
14-3-3 epsilon/YWHAE 研究背景

YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon / YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon / YWHAE is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon / YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon / YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner.

 14-3-3 epsilon/YWHAE 參考資料
  • Ikeda M, et al. (2008) Identification of YWHAE, a gene encoding 14-3-3epsilon, as a possible susceptibility gene for schizophrenia. Hum Mol Genet. 17(20): 3212-22.
  • Mignon-Ravix C, et al. (2010) Deletion of YWHAE in a patient with periventricular heterotopias and pronounced corpus callosum hypoplasia. J Med Genet. 47(2): 132-6.
  • Nagamani SC, et al. (2009) Microdeletions including YWHAE in the Miller-Dieker syndrome region on chromosome 17p13.3 result in facial dysmorphisms, growth restriction, and cognitive impairment. J Med Genet. 46(12): 825-33.
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    貨號: 10842-H09E-100
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”