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人 14-3-3 beta / YWHAB 蛋白

  • Human 14-3-3 beta / YWHAB Protein SDS-PAGE
產品資料評論相關產品實驗方法
 YWHAB 蛋白產品資訊
分子別稱:GW128,HEL-S-1,HS1,KCIP-1,YWHAA
蛋白構建:A DNA sequence encoding the human YWHAB (NP_003395.1) (Met 1-Asn 246) was expressed, with two additional amino acids (Gly & Pro) at the N-terminus.
分子種屬:Human
表達宿主:E. coli
#N/A:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
 YWHAB 蛋白質控檢測
純度:> 93 % as determined by SDS-PAGE
內毒素:Please contact us for more information.
穩定性:Samples are stable for up to twelve months from date of receipt at -70℃
預測N端:Met
分子量:The recombinant human YWHAB consists of 248 amino acids and has a predicted molecular mass of 28 kDa as estimated in SDS-PAGE under reducing conditions.
緩衝液:Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.1mM DTT, 10% glycerol, pH 7.5
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
 YWHAB 蛋白使用指導
儲存方法:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
#N/A:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
 YWHAB Protein SDS-PAGE
Human 14-3-3 beta / YWHAB Protein SDS-PAGE
Other YWHAB Recombinant Protein Products
10843-HNCE
14-3-3 beta/YWHAB 研究背景

14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.

 14-3-3 beta/YWHAB 參考資料
  • Tommerup N, et al. (1996) Assignment of the human genes encoding 14,3-3 Eta (YWHAH) to 22q12, 14-3-3 zeta (YWHAZ) to 2p25.1-p25.2, and 14-3-3 beta (YWHAB) to 20q13.1 by in situ hybridization. Genomics. 33(1): 149-50.
  • Jin YH, et al. (2008) Sirt2 interacts with 14-3-3 beta/gamma and down-regulates the activity of p53. Biochem Biophys Res Commun. 368(3): 690-5.
  • Sekimoto T, et al. (2004) 14-3-3 suppresses the nuclear localization of threonine 157-phosphorylated p27(Kip1). EMBO J. 23(9): 1934-42.
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    貨號: 10843-HNCE-50
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